IL1A ELISA 试剂盒
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- 抗原 See all IL1A ELISA试剂盒
- IL1A (Interleukin 1 alpha (IL1A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.5-300 pg/mL
- 最低检测浓度
- 0.5 pg/mL
- 应用范围
- ELISA
- 原理
- Human IL-1 alpha (IL-1 F1) ELISA Kit for cell and tissue lysate samples.
- 样品类型
- Tissue Lysate, Cell Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human IL-1 alpha.
- 灵敏度
- 0.5 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Cell lysate buffer
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all IL1A ELISA试剂盒
- IL1A (Interleukin 1 alpha (IL1A))
- 别名
- IL-1 alpha (IL1A 产品)
- 别名
- IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit
- 背景
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Gene Names: IL1A IL1F1
Protein names: Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)
- 基因ID
- 3552
- UniProt
- P01583
- 途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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