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HGF ELISA 试剂盒
HGF
适用: 人
Colorimetric
Sandwich ELISA
3-2000 pg/mL
Cell Lysate, Tissue Lysate
HGFELISA Kit详情
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HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
适用
All reactivities for HGF ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
3-2000 pg/mL
最低检测浓度
3 pg/mL
应用范围
ELISA
原理
Human HGF ELISA Kit for cell and tissue lysate samples.
样品类型
Tissue Lysate, Cell Lysate
Analytical Method
Quantitative
特异性
The antibody pair provided in this kit recognizes human HGF.
灵敏度
3 pg/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Cell lysate buffer
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
HGF目标详情
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抗原
See all HGF ELISA试剂盒
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
别名
HGF (HGF 产品 )
别名
DFNB39 ELISA Kit, F-TCF ELISA Kit, HGFB ELISA Kit, HPTA ELISA Kit, SF ELISA Kit, HGF ELISA Kit, C230052L06Rik ELISA Kit, HGF/SF ELISA Kit, NK1 ELISA Kit, NK2 ELISA Kit, SF/HGF ELISA Kit, hepatocyte growth factor ELISA Kit, HGF ELISA Kit, Hgf ELISA Kit
背景
Gene Names: HGF HPTA Protein names: Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain]
基因ID
3082
UniProt
P14210
途径
RTK signaling , Carbohydrate Homeostasis , Glycosaminoglycan Metabolic Process , Synaptic Membrane , Signaling of Hepatocyte Growth Factor Receptor
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