Cyclin B1 ELISA 试剂盒
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- 抗原 See all Cyclin B1 (CCNB1) ELISA试剂盒
- Cyclin B1 (CCNB1)
- 抗原表位
- pSer126, total
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-Cyclin B1 (Ser126) and Total Cyclin B1 ELISA Kit. This assay semi-quantitatively measures Cyclin B1 phosphorylated at Serine-126 as well as total Cyclin B1 in cell lysate samples.
- 样品类型
- Cell Samples, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human Cyclin B1 phosphorylated at site Serine-126 as well as total Cyclin B1.
- 产品特性
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Cyclin B1 (CCNB1) ELISA Kit
CCNB1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Cyclin B1 (CCNB1) ELISA KitCCNB1 适用: 人 pSer126 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Cyclin B1 (CCNB1) ELISA KitCCNB1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cyclin B1 (CCNB1) ELISA KitCCNB1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all Cyclin B1 (CCNB1) ELISA试剂盒
- Cyclin B1 (CCNB1)
- 别名
- Cyclin B1 (CCNB1 产品)
- 别名
- ccnb ELISA Kit, cycb ELISA Kit, cb267 ELISA Kit, cycb1 ELISA Kit, wu:fa19g04 ELISA Kit, wu:fb16d01 ELISA Kit, wu:fb16e07 ELISA Kit, wu:fi21c01 ELISA Kit, ccnb1 ELISA Kit, MGC53596 ELISA Kit, CCNB ELISA Kit, Ccnb1-rs1 ELISA Kit, Ccnb1-rs13 ELISA Kit, CycB1 ELISA Kit, Cycb-4 ELISA Kit, Cycb-5 ELISA Kit, Cycb1-rs1 ELISA Kit, cyclin B1 ELISA Kit, cyclin B1 S homeolog ELISA Kit, ccnb1 ELISA Kit, ccnb1.2 ELISA Kit, ccnb1.S ELISA Kit, CCNB1 ELISA Kit, Ccnb1 ELISA Kit, ccnb1.2.S ELISA Kit
- 基因ID
- 891
- UniProt
- P14635
- 途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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