LTA ELISA 试剂盒
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- 抗原 See all LTA ELISA试剂盒
- LTA (Lymphotoxin-alpha (LTA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- This TNF- enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to TNF- . Standards or samples are then added to the appropriate microtiter plate wells and incubated. After washing to remove unbound TNF- and other components of the sample, biotin-conjugated polyclonal antibody specific to TNF- is added and incubated. If present, TNF- will bind and become immobilized by the antibody pre-coated on the wells and then become
- Analytical Method
- Quantitative
- 灵敏度
- The minimum detectable quantities of human TNF- as observed by the standard curve generated for both Calibrator Diluent I and Calibrator Diluent II are 22.0 pg/mL and 11.0 pg/mL respectively. The two standard deviations above the mean optical density of the 16 replicates of the zero standard were defined as the minimum detectable quantities.
- 组件
- Standards: 1 set/2 vials
- Featured
- Discover our best selling LTA ELISA Kit
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- Discover our top product LTA ELISA Kit
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Lymphotoxin-alpha (LTA) ELISA Kit
LTA 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitSmall Sample LTA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 化学剂 AA 35-205 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 化学剂 Colorimetric Sandwich ELISA 60-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存液
- Without preservative
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- 抗原 See all LTA ELISA试剂盒
- LTA (Lymphotoxin-alpha (LTA))
- 别名
- Tumor Necrosis Factor beta (TNF-b) (LTA 产品)
- 别名
- LT ELISA Kit, TNFB ELISA Kit, TNFSF1 ELISA Kit, LT-[a] ELISA Kit, LT-alpha ELISA Kit, LT[a] ELISA Kit, LTalpha ELISA Kit, Ltx ELISA Kit, TNF-beta ELISA Kit, Tnfb ELISA Kit, Tnfsf1b ELISA Kit, hlb382 ELISA Kit, lymphotoxin alpha ELISA Kit, lymphotoxin A ELISA Kit, LTA ELISA Kit, Lta ELISA Kit
- 物质类
- Chemical
- 背景
- Interleukin-6 (IL-6) is a multifunctional cytokine produced by a wide variety of cell types including monocyte/macrophages, T cells, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas, and glioblastomas. The effects of IL-6 on different cell types are numerous and varied. These activities include: stimulation of B cell differentiation and antibody secretion; action as a co-stimulant with PHA or Con A to increase IL-2 production and IL-2 receptor expression by T cells; enhancement of differentiation of cytotoxic T cells; action as a growth factor for mature thymic or peripheral T cells, myelomas, hybridomas, plasmacytomas, keratinocytes, and mesangial cells; colony-stimulating activity on hematopoetic cells; induction of neuronal cell differentiation; induction of maturation of megakaryocytes; and stimulation of production of acute phase response proteins by hepatocytes. These various activities indicate that IL-6 plays a major role in the mediation of the inflammatory and immune responses initiated by infection or injury. Elevated IL-6 levels have been reported to be associated with a variety of diseases, including autoimmune diseases, mesangial proliferative glomerulonephritis, psoriasis, and malignancies such as plasmacytomas and myelomas. For reviews of the properties and activities of IL-6, see references 1 to 3. The biological activities of IL-6 are initiated by binding of the cytokine to a high affinity receptor complex consisting of two membrane glycoproteins; an 8 kDa component receptor that binds IL-6 with low affinity (IL-6R) and a signal-transducing component of 13 kDa (gp13) that does not bind IL-6 by itself, but is required for high affinity binding of IL-6 by the complex. IL-6R and gp13 have been cloned, sequenced and expressed (4-7). A soluble form of the IL-6 R with a molecular weight of approximately 5 kDa has been found in the urine of healthy adult humans (8), in culture medium conditioned by the growth of a human myeloma cell line (9), in culture supernatants from PHA-stimulated human PBMC and HTLV-1 positive T cell lines (1) and in the serum of HIV-seropositive blood donors (1). This soluble form of the receptor apparently arises from proteolytic cleavage of membrane-bound IL-6R. Soluble forms of human and mouse IL-6 have also been constructed by insertion of termination codons into the regions of the IL-6R cDNAs encoding the external portions of the receptors and prior to the transmembrane domains (11, 12). These soluble receptors have been expressed in COS7 and CHO cells (11, 12) and have been shown to bind IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 sR complex to membrane-bound gp13 (11, 12). The S7.5(3) IL-6 sR 2 _x000C_ regulation in vivo of the shedding of the soluble IL-6Rs and the function and significance of these soluble receptors in biological fluids is not currently understood. It has been suggested, however, that pathogical states involving elevated levels to IL-6 might also be associated with increased production of soluble IL-6Rs (1). This IL-6sR ELISA is a 4.5-hour solid phase immunoassay readily applicable to measure IL-6sR levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of to 2 pg/mL. It showed no cross reactivity with other cytokines tested. This IL-6sR ELISA is expected to be effectively used for further investigations into the relationship between IL-6sR and the various conditions mentioned
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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