IL28B ELISA 试剂盒
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- 抗原 See all IL28B ELISA试剂盒
- IL28B (Interleukin 28B (Interferon, lambda 3) (IL28B))
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适用
- Pig
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Porcine (pig) IL-28B ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Porcine (pig) IL-28B.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 28B (Interferon, lambda 3) (IL28B) ELISA Kit
IL28B 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 28B (Interferon, lambda 3) (IL28B) ELISA KitHigh Sensitivity IL28B 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all IL28B ELISA试剂盒
- IL28B (Interleukin 28B (Interferon, lambda 3) (IL28B))
- 别名
- IL-28B (IL28B 产品)
- 别名
- Il28b ELISA Kit, IFL-1 ELISA Kit, IL-28B ELISA Kit, INF-alpha ELISA Kit, INF-lambda ELISA Kit, Il28 ELISA Kit, IL28B ELISA Kit, IL28C ELISA Kit, interferon, lambda 3 ELISA Kit, interferon lambda 3 ELISA Kit, Ifnl3 ELISA Kit, IFNL3 ELISA Kit
- 基因ID
- 100310828
- UniProt
- D0FHA1
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