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TSC1 ELISA 试剂盒

TSC1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690964
发货至: 中国
  • 抗原 See all TSC1 ELISA试剂盒
    TSC1 (Tuberous Sclerosis 1 (TSC1))
    适用
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human TSC1/Hamartin ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human TSC1.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product TSC1 ELISA Kit
  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all TSC1 ELISA试剂盒
    TSC1 (Tuberous Sclerosis 1 (TSC1))
    别名
    TSC1 (Hamartin (TSC1 产品)
    别名
    LAM ELISA Kit, TSC ELISA Kit, hamartin ELISA Kit, mKIAA0243 ELISA Kit, tsc1 ELISA Kit, wu:fc38b04 ELISA Kit, fa99f04 ELISA Kit, wu:fa99f04 ELISA Kit, zgc:63657 ELISA Kit, TSC complex subunit 1 ELISA Kit, tuberous sclerosis 1 ELISA Kit, TSC complex subunit 1b ELISA Kit, TSC complex subunit 1a ELISA Kit, TSC1 ELISA Kit, Tsc1 ELISA Kit, tsc1b ELISA Kit, tsc1 ELISA Kit, tsc1a ELISA Kit
    基因ID
    7248
    UniProt
    Q92574
    途径
    RTK signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation
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