TSC1 ELISA 试剂盒
TSC1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
抗原
See all TSC1 ELISA试剂盒
-
TSC1
(Tuberous Sclerosis 1 (TSC1))
-
适用
-
人
-
检测方法
-
Colorimetric
-
实验类型
-
Sandwich ELISA
-
应用范围
-
ELISA
-
原理
-
Human TSC1/Hamartin ELISA Kit.
-
样品类型
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
特异性
-
This ELISA antibody pair recognizes Human TSC1.
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
Top Product
-
Discover our top product TSC1 ELISA Kit
-
-
-
板类型
-
Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
-
仅限研究用
-
-
储存条件
-
4 °C
-
有效期
-
6 months
-
-
抗原
See all TSC1 ELISA试剂盒
-
TSC1
(Tuberous Sclerosis 1 (TSC1))
-
别名
-
TSC1 (Hamartin (TSC1 产品)
-
别名
-
LAM ELISA Kit, TSC ELISA Kit, hamartin ELISA Kit, mKIAA0243 ELISA Kit, tsc1 ELISA Kit, wu:fc38b04 ELISA Kit, fa99f04 ELISA Kit, wu:fa99f04 ELISA Kit, zgc:63657 ELISA Kit, TSC complex subunit 1 ELISA Kit, tuberous sclerosis 1 ELISA Kit, TSC complex subunit 1b ELISA Kit, TSC complex subunit 1a ELISA Kit, TSC1 ELISA Kit, Tsc1 ELISA Kit, tsc1b ELISA Kit, tsc1 ELISA Kit, tsc1a ELISA Kit
-
基因ID
-
7248
-
UniProt
-
Q92574
-
途径
-
RTK signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation
-