TRAF3 ELISA 试剂盒
TRAF3
适用: 人
Isoform 2
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all TRAF3 ELISA试剂盒
- TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))
- 抗原表位
- Isoform 2
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human TRAF-3 Isoform 2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human TRAF-3 Isoform 2.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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-
TNF Receptor-Associated Factor 3 (TRAF3) ELISA Kit
TRAF3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
-
- 抗原 See all TRAF3 ELISA试剂盒
- TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))
- 别名
- TRAF-3 (TRAF3 产品)
- 别名
- CAP-1 ELISA Kit, CAP1 ELISA Kit, CD40bp ELISA Kit, CRAF1 ELISA Kit, IIAE5 ELISA Kit, LAP1 ELISA Kit, AI528849 ELISA Kit, T-BAM ELISA Kit, amn ELISA Kit, TNF receptor associated factor 3 ELISA Kit, TNF receptor-associated factor 3 ELISA Kit, Tnf receptor-associated factor 3 ELISA Kit, TRAF3 ELISA Kit, Traf3 ELISA Kit
- 途径
- NF-kappaB Signaling, Apoptosis, TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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