Tryptophan Hydroxylase 1 ELISA 试剂盒
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- 抗原 See all Tryptophan Hydroxylase 1 (TPH1) ELISA试剂盒
- Tryptophan Hydroxylase 1 (TPH1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human TPH-1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human TPH-1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TPH1 ELISA Kit
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all Tryptophan Hydroxylase 1 (TPH1) ELISA试剂盒
- Tryptophan Hydroxylase 1 (TPH1)
- 别名
- TPH-1 (TPH1 产品)
- 别名
- TPRH ELISA Kit, TRPH ELISA Kit, tph ELISA Kit, tph1 ELISA Kit, tphD1 ELISA Kit, tph2 ELISA Kit, tph1l ELISA Kit, tphD2 ELISA Kit, Tph ELISA Kit, TPH ELISA Kit, SERGEF ELISA Kit, LOC100229598 ELISA Kit, tryptophan hydroxylase 1 ELISA Kit, tryptophan hydroxylase 1 (tryptophan 5-monooxygenase) a ELISA Kit, TryPtophan Hydroxylase ELISA Kit, tryptophan hydroxylase 1b ELISA Kit, tryptophan hydroxylase 1 L homeolog ELISA Kit, tryptophan 5-hydroxylase 1 ELISA Kit, TPH1 ELISA Kit, tph1a ELISA Kit, tph-1 ELISA Kit, tph1b ELISA Kit, Tph1 ELISA Kit, tph1.L ELISA Kit, LOAG_02149 ELISA Kit, Tsp_05203 ELISA Kit
- 基因ID
- 7166
- UniProt
- P17752
- 途径
- Feeding Behaviour
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