TCF7L1 ELISA 试剂盒
TCF7L1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all TCF7L1 ELISA试剂盒
- TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human TCF7L1/TCF-3 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human TCF7L1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all TCF7L1 ELISA试剂盒
- TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))
- 别名
- TCF7L1 (TCF-3 (TCF7L1 产品)
- 别名
- TCF-3 ELISA Kit, TCF3 ELISA Kit, Tcf-3 ELISA Kit, Tcf3 ELISA Kit, bHLHb21 ELISA Kit, tcf3c ELISA Kit, tcf3d ELISA Kit, xtcf3 ELISA Kit, XTCF-3 ELISA Kit, tcf7l1 ELISA Kit, tcf3b ELISA Kit, TCF-3-A ELISA Kit, tcf-3 ELISA Kit, tcf3 ELISA Kit, tcf3a ELISA Kit, zTcf-3 ELISA Kit, transcription factor 7 like 1 ELISA Kit, transcription factor 7 like 1 (T cell specific, HMG box) ELISA Kit, transcription factor 7 like 1 L homeolog ELISA Kit, transcription factor 7 like 1 S homeolog ELISA Kit, transcription factor 7 like 1b ELISA Kit, transcription factor 7 like 1a ELISA Kit, TCF7L1 ELISA Kit, Tcf7l1 ELISA Kit, tcf7l1.L ELISA Kit, tcf7l1.S ELISA Kit, tcf7l1b ELISA Kit, tcf7l1a ELISA Kit
- 基因ID
- 83439
- UniProt
- Q9HCS4
- 途径
- WNT signaling, Stem Cell Maintenance
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