SOX21 ELISA 试剂盒
SOX21
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all SOX21 products
- SOX21 (SRY (Sex Determining Region Y)-Box 21 (SOX21))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human SOX21/SOX-A ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human SOX21.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all SOX21 products
- SOX21 (SRY (Sex Determining Region Y)-Box 21 (SOX21))
- 别名
- SOX21 (SOX-A (SOX21 产品)
- 别名
- SOX25 ELISA Kit, Sox25 ELISA Kit, CG7345 ELISA Kit, Dmel\\CG7345 ELISA Kit, SOXB2.3 ELISA Kit, Sox B2-3 ELISA Kit, Sox21 ELISA Kit, SoxB2.3 ELISA Kit, sox25 ELISA Kit, SRY-box ELISA Kit, sox21 ELISA Kit, sox30 ELISA Kit, zgc:110678 ELISA Kit, CH31 ELISA Kit, SRY-box 21 ELISA Kit, SRY (sex determining region Y)-box 21 ELISA Kit, SRY-box 21 S homeolog ELISA Kit, CG7345 gene product from transcript CG7345-RA ELISA Kit, SRY box 21 ELISA Kit, SRY (sex determining region Y)-box 21a ELISA Kit, SRY (sex determining region Y)-box 21b ELISA Kit, SOX21 ELISA Kit, Sox21 ELISA Kit, sox21.S ELISA Kit, Sox21a ELISA Kit, sox21 ELISA Kit, sox21a ELISA Kit, sox21b ELISA Kit
- 基因ID
- 11166
- UniProt
- Q9Y651
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