PAG1 ELISA 试剂盒
PAG1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all PAG1 ELISA试剂盒
- PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human PAG1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human PAG1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
-
- 抗原 See all PAG1 ELISA试剂盒
- PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
- 别名
- PAG1 (PAG1 产品)
- 别名
- Cbp ELISA Kit, F730007C19Rik ELISA Kit, Pag ELISA Kit, CBP ELISA Kit, PAG ELISA Kit, MGC80192 ELISA Kit, phosphoprotein associated with glycosphingolipid microdomains 1 ELISA Kit, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 ELISA Kit, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog ELISA Kit, Pag1 ELISA Kit, PAG1 ELISA Kit, pag1.L ELISA Kit, pag1 ELISA Kit
- 基因ID
- 55824
- UniProt
- Q9NWQ8
- 途径
- p53 Pathway, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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