BCAR1 ELISA 试剂盒
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- 抗原 See all BCAR1 ELISA试剂盒
- BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human p130Cas/BCAR1/CAS ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human p130Cas.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) ELISA Kit
BCAR1 适用: 人 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) ELISA KitBCAR1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all BCAR1 ELISA试剂盒
- BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))
- 别名
- p130Cas (BCAR1/CAS) (BCAR1 产品)
- 别名
- CAS ELISA Kit, CAS1 ELISA Kit, CASS1 ELISA Kit, CRKAS ELISA Kit, P130Cas ELISA Kit, AI385681 ELISA Kit, Cas ELISA Kit, Crkas ELISA Kit, P130CAS ELISA Kit, MGC68466 ELISA Kit, cas ELISA Kit, p130cas ELISA Kit, BCAR1 ELISA Kit, fc17f11 ELISA Kit, wu:fc17f11 ELISA Kit, zgc:175192 ELISA Kit, BCAR1, Cas family scaffolding protein ELISA Kit, breast cancer anti-estrogen resistance 1 ELISA Kit, breast cancer anti-estrogen resistance 1 L homeolog ELISA Kit, breast cancer anti-estrogen resistance protein 1 ELISA Kit, BCAR1 ELISA Kit, Bcar1 ELISA Kit, bcar1.L ELISA Kit, bcar1 ELISA Kit, LOC100435586 ELISA Kit
- 基因ID
- 9564
- UniProt
- P56945
- 途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling
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