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NEDD8 ELISA 试剂盒

NEDD8 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690916
发货至: 中国
  • 抗原 See all NEDD8 ELISA试剂盒
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    适用
    • 2
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human NEDD8 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human NEDD8.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product NEDD8 ELISA Kit
  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all NEDD8 ELISA试剂盒
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    别名
    NEDD8 (NEDD8 产品)
    别名
    NEDD-8 ELISA Kit, Rub1 ELISA Kit, CG10679 ELISA Kit, Dmel\\CG10679 ELISA Kit, NEDD8 ELISA Kit, dNEDD8 ELISA Kit, nedd8 ELISA Kit, ubiquitin ELISA Kit, si:zc14a17.5 ELISA Kit, GB17785 ELISA Kit, DDBDRAFT_0218116 ELISA Kit, DDBDRAFT_0238041 ELISA Kit, DDB_0218116 ELISA Kit, DDB_0238041 ELISA Kit, nedd8.S ELISA Kit, neural precursor cell expressed, developmentally down-regulated 8 ELISA Kit, neural precursor cell expressed, developmentally down-regulated gene 8 ELISA Kit, CG10679 gene product from transcript CG10679-RB ELISA Kit, Nedd8 ELISA Kit, ubiquitin-like protein Nedd8 ELISA Kit, neural precursor cell expressed, developmentally down-regulated 8 L homeolog ELISA Kit, NEDD8 ELISA Kit, Nedd8 ELISA Kit, nedd8 ELISA Kit, nedd8.L ELISA Kit
    基因ID
    4738
    UniProt
    Q15843
    途径
    Ubiquitin Proteasome Pathway
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