Myogenin ELISA 试剂盒
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- 抗原 See all Myogenin (MYOG) ELISA试剂盒
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Myogenin ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Myogenin.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling MYOG ELISA Kit
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all Myogenin (MYOG) ELISA试剂盒
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
- 别名
- Myogenin (MYOG 产品)
- 别名
- cb553 ELISA Kit, zgc:100763 ELISA Kit, LOC446037 ELISA Kit, myf4 ELISA Kit, Xmyog ELISA Kit, myogenin ELISA Kit, LOC100136471 ELISA Kit, LOC100224062 ELISA Kit, LOC100303673 ELISA Kit, MYF4 ELISA Kit, bHLHc3 ELISA Kit, myo ELISA Kit, myf-4 ELISA Kit, myogenin ELISA Kit, myogenin (myogenic factor 4) ELISA Kit, MYOG ELISA Kit, myog ELISA Kit, LOC100303673 ELISA Kit, Myog ELISA Kit
- 基因ID
- 4656
- UniProt
- P15173
- 途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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