JNK2 ELISA 试剂盒
MAPK9
适用: 人
Isoform alpha-1
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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抗原
See all JNK2 (MAPK9) ELISA试剂盒
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JNK2 (MAPK9)
(Mitogen-Activated Protein Kinase 9 (MAPK9))
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抗原表位
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Isoform alpha-1
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适用
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人
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检测方法
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Colorimetric
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实验类型
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Sandwich ELISA
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应用范围
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ELISA
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原理
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Human JNK2/MAPK9 isoform alpha-1 ELISA Kit.
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样品类型
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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特异性
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This ELISA antibody pair recognizes Human JNK2.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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板类型
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Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
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仅限研究用
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储存条件
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4 °C
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有效期
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6 months
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抗原
See all JNK2 (MAPK9) ELISA试剂盒
-
JNK2 (MAPK9)
(Mitogen-Activated Protein Kinase 9 (MAPK9))
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别名
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JNK2 (MAPK9 (MAPK9 产品)
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别名
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MAPK9 ELISA Kit, JNK-55 ELISA Kit, JNK2 ELISA Kit, JNK2A ELISA Kit, JNK2ALPHA ELISA Kit, JNK2B ELISA Kit, JNK2BETA ELISA Kit, PRKM9 ELISA Kit, SAPK ELISA Kit, SAPK1a ELISA Kit, p54a ELISA Kit, p54aSAPK ELISA Kit, AI851083 ELISA Kit, Prkm9 ELISA Kit, JNK2-ALPHA1 ELISA Kit, MAP kinase 9 ELISA Kit, mitogen-activated protein kinase 9 ELISA Kit, MAP kinase 9 ELISA Kit, MAPK9 ELISA Kit, Mapk9 ELISA Kit, MPK9 ELISA Kit
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途径
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MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins
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