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ILKAP ELISA 试剂盒

ILKAP 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690894
发货至: 中国
  • 抗原 See all ILKAP ELISA试剂盒
    ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))
    适用
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human ILKAP ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human ILKAP.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ILKAP ELISA Kit
  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all ILKAP ELISA试剂盒
    ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))
    别名
    ILKAP (ILKAP 产品)
    别名
    MGC84595 ELISA Kit, MGC107938 ELISA Kit, ILKAP ELISA Kit, ILKAP2 ELISA Kit, ILKAP3 ELISA Kit, PP2C-DELTA ELISA Kit, 0710007A14Rik ELISA Kit, 1600009O09Rik ELISA Kit, ILK associated serine/threonine phosphatase ELISA Kit, ILK associated serine/threonine phosphatase L homeolog ELISA Kit, integrin-linked kinase-associated serine/threonine phosphatase ELISA Kit, integrin-linked kinase-associated serine/threonine phosphatase 2C ELISA Kit, ILKAP ELISA Kit, ilkap.L ELISA Kit, ilkap ELISA Kit, Ilkap ELISA Kit
    基因ID
    80895
    UniProt
    Q9H0C8
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