WFDC2 ELISA 试剂盒
-
- 抗原 See all WFDC2 ELISA试剂盒
- WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human HE4/WFDC2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human HE4.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling WFDC2 ELISA Kit
- Top Product
- Discover our top product WFDC2 ELISA Kit
-
-
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 有效期
- 6 months
-
-
Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA)." in: Journal of clinical laboratory analysis, Vol. 30, Issue 5, pp. 581-9, (2017) (PubMed).
: "
-
Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA)." in: Journal of clinical laboratory analysis, Vol. 30, Issue 5, pp. 581-9, (2017) (PubMed).
-
- 抗原 See all WFDC2 ELISA试剂盒
- WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))
- 别名
- HE4 (WFDC2 (WFDC2 产品)
- 别名
- WFDC3 ELISA Kit, WFDC2 ELISA Kit, si:ch211-153a8.3 ELISA Kit, EDDM4 ELISA Kit, HE4 ELISA Kit, WAP5 ELISA Kit, dJ461P17.6 ELISA Kit, 1600023A02Rik ELISA Kit, re4 ELISA Kit, CE4 ELISA Kit, BE-20 ELISA Kit, WAP four-disulfide core domain 2 ELISA Kit, WFDC2 ELISA Kit, wfdc2 ELISA Kit, Wfdc2 ELISA Kit
- 基因ID
- 10406
- UniProt
- Q14508
-