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HAPLN4 ELISA 试剂盒

HAPLN4 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690887
发货至: 中国
  • 抗原 See all HAPLN4 ELISA试剂盒
    HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human HAPLN4 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human HAPLN4.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all HAPLN4 ELISA试剂盒
    HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4))
    别名
    HAPLN4 (HAPLN4 产品)
    别名
    HAPLN4 ELISA Kit, si:dkey-19f23.2 ELISA Kit, hpln4 ELISA Kit, BRAL2 ELISA Kit, 9330174O11 ELISA Kit, Bral2 ELISA Kit, Lpr4 ELISA Kit, hyaluronan and proteoglycan link protein 4 ELISA Kit, Hyaluronan and proteoglycan link protein 4 ELISA Kit, Hapln4 ELISA Kit, HAPLN4 ELISA Kit, hapln4 ELISA Kit, hpln4 ELISA Kit
    基因ID
    404037
    UniProt
    Q86UW8
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