GMFB ELISA 试剂盒
GMFB
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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抗原
See all GMFB ELISA试剂盒
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GMFB
(Glia Maturation Factor, beta (GMFB))
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适用
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人
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检测方法
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Colorimetric
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实验类型
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Sandwich ELISA
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应用范围
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ELISA
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原理
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Human GMF-beta ELISA Kit.
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样品类型
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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特异性
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This ELISA antibody pair recognizes Human GMF-beta.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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板类型
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Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
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仅限研究用
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储存条件
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4 °C
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有效期
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6 months
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抗原
See all GMFB ELISA试剂盒
-
GMFB
(Glia Maturation Factor, beta (GMFB))
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别名
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GMF-beta (GMFB 产品)
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别名
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fc25b01 ELISA Kit, zgc:56451 ELISA Kit, zgc:86831 ELISA Kit, wu:fa97c03 ELISA Kit, wu:fc25b01 ELISA Kit, GMFB ELISA Kit, DKFZp459J101 ELISA Kit, gmfb ELISA Kit, 3110001H22Rik ELISA Kit, 3110001O16Rik ELISA Kit, AI851627 ELISA Kit, C79176 ELISA Kit, D14Ertd630e ELISA Kit, GMF ELISA Kit, gmf-beta ELISA Kit, glia maturation factor, beta ELISA Kit, glia maturation factor beta ELISA Kit, Glia maturation factor beta ELISA Kit, glia maturation factor, beta L homeolog ELISA Kit, gmfb ELISA Kit, GMFB ELISA Kit, Gmfb ELISA Kit, EDI_093190 ELISA Kit, Tsp_04922 ELISA Kit, LOC100101176 ELISA Kit, gmfb.L ELISA Kit
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基因ID
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2764
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UniProt
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P60983
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