Caspase 1 ELISA 试剂盒
-
- 抗原 See all Caspase 1 (CASP1) ELISA试剂盒
- Caspase 1 (CASP1)
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Caspase-1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Caspase-1.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling CASP1 ELISA Kit
- Top Product
- Discover our top product CASP1 ELISA Kit
-
-
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 有效期
- 6 months
-
- 抗原 See all Caspase 1 (CASP1) ELISA试剂盒
- Caspase 1 (CASP1)
- 别名
- Caspase-1 (CASP1 产品)
- 别名
- ICE ELISA Kit, IL1BC ELISA Kit, P45 ELISA Kit, Il1bc ELISA Kit, Ice ELISA Kit, CASP1 ELISA Kit, CG5370 ELISA Kit, DCP-1 ELISA Kit, DCP1 ELISA Kit, Dcp1 ELISA Kit, Dmel\\CG5370 ELISA Kit, ccp1 ELISA Kit, dcp-1 ELISA Kit, dcp1 ELISA Kit, l(2)01862 ELISA Kit, l(2)02132 ELISA Kit, caspase 1 ELISA Kit, caspase 1, apoptosis-related cysteine peptidase ELISA Kit, caspase 4, apoptosis-related cysteine peptidase ELISA Kit, Death caspase-1 ELISA Kit, caspase-1 ELISA Kit, caspase ELISA Kit, caspase recruitment domain-containing protein 16 ELISA Kit, caspase 4 ELISA Kit, CASP1 ELISA Kit, Casp1 ELISA Kit, CASP4 ELISA Kit, Dcp-1 ELISA Kit, Caspase1 ELISA Kit, LOC5564196 ELISA Kit, LOC451519 ELISA Kit
- 基因ID
- 834
- UniProt
- P29466
- 途径
- Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome
-