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T Brachyury Protein ELISA 试剂盒

适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690832
发货至: 中国
  • 抗原 See all T Brachyury Protein products
    T Brachyury Protein
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Brachyury/Protein T ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human Brachyury.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all T Brachyury Protein products
    T Brachyury Protein
    别名
    Brachyury (Protein T (T Brachyury Protein 产品)
    别名
    Bra ELISA Kit, D17Mit170 ELISA Kit, Low ELISA Kit, Lr ELISA Kit, T1 ELISA Kit, Tl2 ELISA Kit, Tl3 ELISA Kit, cou ELISA Kit, me75 ELISA Kit, X-bra ELISA Kit, Xbrachyury ELISA Kit, bra ELISA Kit, brachyury ELISA Kit, bu ELISA Kit, ntl ELISA Kit, t ELISA Kit, ta ELISA Kit, xbra ELISA Kit, TFT ELISA Kit, CH-T ELISA Kit, brachyury ELISA Kit, T brachyury transcription factor ELISA Kit, T brachyury transcription factor S homeolog ELISA Kit, T ELISA Kit, t.S ELISA Kit
    基因ID
    6862
    UniProt
    O15178
    途径
    Tube Formation
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