T Brachyury Protein ELISA 试剂盒
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all T Brachyury Protein products
- T Brachyury Protein
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Brachyury/Protein T ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Brachyury.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all T Brachyury Protein products
- T Brachyury Protein
- 别名
- Brachyury (Protein T (T Brachyury Protein 产品)
- 别名
- Bra ELISA Kit, D17Mit170 ELISA Kit, Low ELISA Kit, Lr ELISA Kit, T1 ELISA Kit, Tl2 ELISA Kit, Tl3 ELISA Kit, cou ELISA Kit, me75 ELISA Kit, X-bra ELISA Kit, Xbrachyury ELISA Kit, bra ELISA Kit, brachyury ELISA Kit, bu ELISA Kit, ntl ELISA Kit, t ELISA Kit, ta ELISA Kit, xbra ELISA Kit, TFT ELISA Kit, CH-T ELISA Kit, brachyury ELISA Kit, T brachyury transcription factor ELISA Kit, T brachyury transcription factor S homeolog ELISA Kit, T ELISA Kit, t.S ELISA Kit
- 基因ID
- 6862
- UniProt
- O15178
- 途径
- Tube Formation
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