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APP ELISA 试剂盒

APP 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690821
发货至: 中国
  • 抗原 See all APP ELISA试剂盒
    APP (Amyloid beta (A4) Precursor Protein (APP))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Amyloid-beta A4 protein/APP/PN-II ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human Amyloid-beta A4 protein.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all APP ELISA试剂盒
    APP (Amyloid beta (A4) Precursor Protein (APP))
    别名
    Amyloid-beta A4 protein (APP/PN-II) (APP 产品)
    别名
    AAA ELISA Kit, ABETA ELISA Kit, ABPP ELISA Kit, AD1 ELISA Kit, APPI ELISA Kit, CTFgamma ELISA Kit, CVAP ELISA Kit, PN-II ELISA Kit, PN2 ELISA Kit, aaa ELISA Kit, abeta ELISA Kit, abpp ELISA Kit, ad1 ELISA Kit, appi ELISA Kit, ctfgamma ELISA Kit, cvap ELISA Kit, pn2 ELISA Kit, APP ELISA Kit, APP-like ELISA Kit, APPL ELISA Kit, Abeta ELISA Kit, BcDNA:GH04413 ELISA Kit, CG7727 ELISA Kit, Dmel\\CG7727 ELISA Kit, EG:65F1.5 ELISA Kit, appl ELISA Kit, Abpp ELISA Kit, Adap ELISA Kit, Ag ELISA Kit, Cvap ELISA Kit, E030013M08Rik ELISA Kit, betaApp ELISA Kit, app ELISA Kit, wu:fj34d10 ELISA Kit, wu:fk65e12 ELISA Kit, zgc:85740 ELISA Kit, amyloid beta precursor protein ELISA Kit, amyloid beta (A4) precursor protein ELISA Kit, beta amyloid protein precursor-like ELISA Kit, amyloid beta (A4) precursor protein a ELISA Kit, amyloid beta precursor protein L homeolog ELISA Kit, APP ELISA Kit, app ELISA Kit, Appl ELISA Kit, App ELISA Kit, appa ELISA Kit, app.L ELISA Kit
    基因ID
    351
    UniProt
    P05067
    途径
    Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
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