APP ELISA 试剂盒
APP
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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抗原
See all APP ELISA试剂盒
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APP
(Amyloid beta (A4) Precursor Protein (APP))
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适用
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人
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检测方法
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Colorimetric
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实验类型
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Sandwich ELISA
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应用范围
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ELISA
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原理
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Human Amyloid-beta A4 protein/APP/PN-II ELISA Kit.
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样品类型
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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特异性
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This ELISA antibody pair recognizes Human Amyloid-beta A4 protein.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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板类型
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Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
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仅限研究用
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储存条件
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4 °C
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有效期
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6 months
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抗原
See all APP ELISA试剂盒
-
APP
(Amyloid beta (A4) Precursor Protein (APP))
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别名
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Amyloid-beta A4 protein (APP/PN-II) (APP 产品)
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别名
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AAA ELISA Kit, ABETA ELISA Kit, ABPP ELISA Kit, AD1 ELISA Kit, APPI ELISA Kit, CTFgamma ELISA Kit, CVAP ELISA Kit, PN-II ELISA Kit, PN2 ELISA Kit, aaa ELISA Kit, abeta ELISA Kit, abpp ELISA Kit, ad1 ELISA Kit, appi ELISA Kit, ctfgamma ELISA Kit, cvap ELISA Kit, pn2 ELISA Kit, APP ELISA Kit, APP-like ELISA Kit, APPL ELISA Kit, Abeta ELISA Kit, BcDNA:GH04413 ELISA Kit, CG7727 ELISA Kit, Dmel\\CG7727 ELISA Kit, EG:65F1.5 ELISA Kit, appl ELISA Kit, Abpp ELISA Kit, Adap ELISA Kit, Ag ELISA Kit, Cvap ELISA Kit, E030013M08Rik ELISA Kit, betaApp ELISA Kit, app ELISA Kit, wu:fj34d10 ELISA Kit, wu:fk65e12 ELISA Kit, zgc:85740 ELISA Kit, amyloid beta precursor protein ELISA Kit, amyloid beta (A4) precursor protein ELISA Kit, beta amyloid protein precursor-like ELISA Kit, amyloid beta (A4) precursor protein a ELISA Kit, amyloid beta precursor protein L homeolog ELISA Kit, APP ELISA Kit, app ELISA Kit, Appl ELISA Kit, App ELISA Kit, appa ELISA Kit, app.L ELISA Kit
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基因ID
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351
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UniProt
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P05067
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途径
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Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
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