PPARG ELISA 试剂盒
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- 抗原 See all PPARG ELISA试剂盒
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- Human PPAR-gamma Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical PPAR-gamma binding sequences to semi-quantitatively detect active PPAR-gamma in lysates or nuclear extracts.
- 样品类型
- Cell Lysate, Nuclear Extract
- Analytical Method
- Semi-Quantitative
- 特异性
- The olionucleotide/antibody pair provided in this kit recognizes human PPAR-gamma in whole lysates and nuclear extracts.
- 产品特性
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- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
- 组件
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- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our top product PPARG ELISA Kit
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Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA Kit
PPARG 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 兔 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Peroxisome Proliferator-Activated Receptor gamma (PPARG) ELISA KitPPARG 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
-
- 抗原 See all PPARG ELISA试剂盒
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
- 别名
- PPAR-gamma (PPARG 产品)
- 别名
- PPAR gamma ELISA Kit, PPARg2 ELISA Kit, PPARG ELISA Kit, Nr1c3 ELISA Kit, PPAR-gamma ELISA Kit, PPAR-gamma2 ELISA Kit, PPARgamma ELISA Kit, PPARgamma2 ELISA Kit, NR1C3 ELISA Kit, CIMT1 ELISA Kit, GLM1 ELISA Kit, PPARG1 ELISA Kit, PPARG2 ELISA Kit, xPPAR-gamma ELISA Kit, PPAR-GAMMA ELISA Kit, PPARGAMMA ELISA Kit, peroxisome proliferator-activated receptor gamma ELISA Kit, peroxisome proliferator activated receptor gamma ELISA Kit, peroxisome proliferator activated receptor gamma L homeolog ELISA Kit, PPARG ELISA Kit, Pparg ELISA Kit, pparg.L ELISA Kit
- 基因ID
- 5468
- UniProt
- P37231
- 途径
- MAPK Pathway, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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