GATA1 ELISA 试剂盒
GATA1
适用: 人
Colorimetric
DNA-Binding ELISA
Cell Lysate, Nuclear Extract
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- 抗原 See all GATA1 ELISA试剂盒
- GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- Human GATA-1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA1 binding sequences to semi-quantitatively detect active GATA1 in lysates or nuclear extracts.
- 样品类型
- Cell Lysate, Nuclear Extract
- Analytical Method
- Semi-Quantitative
- 特异性
- The olionucleotide/antibody pair provided in this kit recognizes human GATA-1 in whole lysates and nuclear extracts.
- 产品特性
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- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
- 组件
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- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
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- 抗原 See all GATA1 ELISA试剂盒
- GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
- 别名
- GATA1 (GATA1 产品)
- 别名
- ERYF1 ELISA Kit, GATA-1 ELISA Kit, GF-1 ELISA Kit, GF1 ELISA Kit, NF-E1 ELISA Kit, NFE1 ELISA Kit, XLANP ELISA Kit, XLTDA ELISA Kit, XLTT ELISA Kit, Gata-1 ELISA Kit, Gf-1 ELISA Kit, eryf1 ELISA Kit, Eryf1 ELISA Kit, GATA1 ELISA Kit, gata-1 ELISA Kit, Gata1 ELISA Kit, si:dkeyp-77f7.5 ELISA Kit, gata1 ELISA Kit, gata1a-A ELISA Kit, xGATA-1 ELISA Kit, xgata1 ELISA Kit, XGATA-1B ELISA Kit, gata1b-A ELISA Kit, gta1 ELISA Kit, zg1 ELISA Kit, NF-E1a ELISA Kit, GATA binding protein 1 ELISA Kit, GATA binding protein 1 (globin transcription factor 1) ELISA Kit, amidase ELISA Kit, aspartyl/glutamyl-tRNA amidotransferase subunit A ELISA Kit, putative amidase ELISA Kit, GatA1 ELISA Kit, glutamyl-tRNA amidotransferase subunit A ELISA Kit, hematopietic transcription factor GATA-1 ELISA Kit, GATA binding protein 1b ELISA Kit, GATA binding protein 1 L homeolog ELISA Kit, GATA binding protein 1 S homeolog ELISA Kit, GATA binding protein 1a ELISA Kit, erythroid transcription factor ELISA Kit, GATA1 ELISA Kit, Gata1 ELISA Kit, gatA1 ELISA Kit, gata-1 ELISA Kit, gata1b ELISA Kit, gata1.L ELISA Kit, gata1.S ELISA Kit, gata1a ELISA Kit, LOC108348091 ELISA Kit
- 基因ID
- 2623
- UniProt
- P15976
- 途径
- Cellular Response to Molecule of Bacterial Origin
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