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Estrogen Receptor alpha ELISA 试剂盒

Name: Estrogen Receptor alpha ELISA 试剂盒
SKU: ABIN5690752
Availability: OutOfStock

ESR1 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract

Transcription factor assay of ER-alpha from nuclear extracts of MCF-7 cells or MCF-7 cells treated with H2O2 (200uM) for 3 hr with the specific competitor or non-specific competitor. (Estrogen Receptor alpha ELISA 试剂盒)

AcA

Transcription factor assay of ER-alpha from nuclear extracts of MCF-7 cells or MCF-7 cells treated with H2O2 (200uM) for 3 hr with Activity Assay Kit. (Estrogen Receptor alpha ELISA 试剂盒)

AcA

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产品编号 ABIN5690752

发货至: 中国

Datasheet as PDF

抗原 See all Estrogen Receptor alpha (ESR1) ELISA试剂盒

Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
适用
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人

检测方法

Colorimetric

实验类型

DNA-Binding ELISA

应用范围

ELISA

原理

Human ER-alpha Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical ER-alpha binding sequences to semi-quantitatively detect active ER-alpha in lysates or nuclear extracts.

样品类型

Cell Lysate, Nuclear Extract

Analytical Method

Semi-Quantitative

特异性

The olionucleotide/antibody pair provided in this kit recognizes mouse ER-alpha in whole lysates and nuclear extracts.

产品特性
- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
组件
- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
试剂未包括
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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ESR1 适用: 人 Colorimetric DNA-Binding ELISA Cell Extracts, Tissue Samples

板类型

Pre-coated

实验流程
1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

4 °C

储存方法

Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

有效期

6 months
抗原 See all Estrogen Receptor alpha (ESR1) ELISA试剂盒

Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

别名

ER-alpha (ESR1 产品)

别名

LOC398734 ELISA Kit, AA420328 ELISA Kit, AU041214 ELISA Kit, ER-alpha ELISA Kit, ER[a] ELISA Kit, ERa ELISA Kit, ERalpha ELISA Kit, ESR ELISA Kit, Estr ELISA Kit, Estra ELISA Kit, Nr3a1 ELISA Kit, NR3A1 ELISA Kit, eralpha ELISA Kit, zfER[a] ELISA Kit, ER ELISA Kit, ESRA ELISA Kit, ESTRR ELISA Kit, Era ELISA Kit, akap12 ELISA Kit, cb27 ELISA Kit, esr1 ELISA Kit, fb72g12 ELISA Kit, id:ibd1202 ELISA Kit, sb:cb27 ELISA Kit, si:ch73-192g21.1 ELISA Kit, wu:fb72g12 ELISA Kit, wu:fc21c03 ELISA Kit, Esr ELISA Kit, RNESTROR ELISA Kit, ERALPHA ELISA Kit, er ELISA Kit, esr ELISA Kit, nr3a1 ELISA Kit, XER ELISA Kit, era ELISA Kit, esra ELISA Kit, ERbeta ELISA Kit, xesr-1 ELISA Kit, xlERalpha1 ELISA Kit, xlERalpha2 ELISA Kit, ESR1 ELISA Kit, estrogen receptor 1 L homeolog ELISA Kit, estrogen receptor 1 (alpha) ELISA Kit, estrogen receptor 1 ELISA Kit, A kinase (PRKA) anchor protein 12b ELISA Kit, esr1.L ELISA Kit, Esr1 ELISA Kit, ESR1 ELISA Kit, esr1 ELISA Kit, akap12b ELISA Kit

基因ID

2099

UniProt

P03372

途径

Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly