c-MYC ELISA 试剂盒
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- 抗原 See all c-MYC (MYC) ELISA试剂盒
- c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- Human c-Myc Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical c-Myc binding sequences to semi-quantitatively detect active c-Myc in lysates or nuclear extracts.
- 样品类型
- Cell Lysate, Nuclear Extract
- Analytical Method
- Semi-Quantitative
- 特异性
- The olionucleotide/antibody pair provided in this kit recognizes human TFEB in whole lysates and nuclear extracts.
- 产品特性
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- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
- 组件
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- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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Myc Proto-Oncogene protein (MYC) ELISA Kit
MYC 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myc Proto-Oncogene protein (MYC) ELISA KitMYC 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myc Proto-Oncogene protein (MYC) ELISA KitMYC 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Myc Proto-Oncogene protein (MYC) ELISA KitMYC 适用: 人 Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
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- 抗原 See all c-MYC (MYC) ELISA试剂盒
- c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))
- 别名
- c-Myc (MYC 产品)
- 别名
- MRTL ELISA Kit, MYCC ELISA Kit, bHLHe39 ELISA Kit, c-Myc ELISA Kit, CMYC ELISA Kit, c-myc ELISA Kit, C-MYC ELISA Kit, LOC100136746 ELISA Kit, ARC ELISA Kit, B430311C09Rik ELISA Kit, MYC ELISA Kit, NOP ELISA Kit, Nop30 ELISA Kit, RNCMYC ELISA Kit, mMyc ELISA Kit, AU016757 ELISA Kit, Myc2 ELISA Kit, Niard ELISA Kit, Nird ELISA Kit, cmyc ELISA Kit, zc-myc ELISA Kit, c-myc II ELISA Kit, myc-B ELISA Kit, myc2 ELISA Kit, myc1 ELISA Kit, CMyc ELISA Kit, zgc:55680 ELISA Kit, CG10798 ELISA Kit, D-Myc ELISA Kit, DM ELISA Kit, DMYc ELISA Kit, Dm ELISA Kit, Dmel\CG10798 ELISA Kit, Dmyc ELISA Kit, EG:BACN5I9.1 ELISA Kit, anon-WO03040301.171 ELISA Kit, bHLHe57 ELISA Kit, c-MYC ELISA Kit, d-myc ELISA Kit, dMYC ELISA Kit, dMyc ELISA Kit, dMyc1 ELISA Kit, da ELISA Kit, dm ELISA Kit, dm/dMyc ELISA Kit, dm/myc ELISA Kit, dmyc ELISA Kit, dmyc1 ELISA Kit, l(1)G0139 ELISA Kit, l(1)G0354 ELISA Kit, l(1)G0359 ELISA Kit, myc ELISA Kit, MYC proto-oncogene, bHLH transcription factor ELISA Kit, v-myc myelocytomatosis viral oncogene homolog ELISA Kit, nucleolar protein 3 (apoptosis repressor with CARD domain) ELISA Kit, myelocytomatosis oncogene ELISA Kit, MYC proto-oncogene, bHLH transcription factor a ELISA Kit, MYC proto-oncogene, bHLH transcription factor L homeolog ELISA Kit, MYC proto-oncogene, bHLH transcription factor S homeolog ELISA Kit, v-myc myelocytomatosis viral oncogene homolog (avian) ELISA Kit, MYC proto-oncogene, bHLH transcription factor b ELISA Kit, CG10798 gene product from transcript CG10798-RB ELISA Kit, MYC ELISA Kit, LOC100136746 ELISA Kit, Nol3 ELISA Kit, Myc ELISA Kit, myca ELISA Kit, myc.L ELISA Kit, myc.S ELISA Kit, mycb ELISA Kit
- 基因ID
- 4609
- UniProt
- P01106
- 途径
- p53 Pathway, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect
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