RPS6KA3 ELISA 试剂盒
RPS6KA3
适用: 人, 小鼠, 大鼠
Colorimetric
Cell ELISA
Adherent Cell Culture
-
- 抗原 See all RPS6KA3 ELISA试剂盒
- RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
- 适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Cell ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse and Rat Phospho-RSK2 (Ser386) and Total RSK2 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures RSK2 phosphorylated at Serine-386 as well as total RSK2 in adherent cell lines.
- 样品类型
- Adherent Cell Culture
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibodies provided in this kit recognizes Human, Mouse and Rat RSK2 phosphorylated at site Serine-386 as well as total RSK2.
- 产品特性
-
- Rapidly measure phosphorylated protein in adherent cell lines
- Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
- No sample lysis is needed
- Compatible with a standard ELISA plate reader
- 组件
-
- Uncoated 96-well Strip Microplate
- Wash Buffers
- Fixing Solution
- Quenching Buffer
- Blocking Buffer
- Anti-phospho antibody
- Anti-pan antibody
- HRP-Conjugated Secondary Antibody
- TMB One-Step Substrate
- Stop Solution
- 试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product RPS6KA3 ELISA Kit
-
-
- 板类型
- Uncoated
- 实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all RPS6KA3 ELISA试剂盒
- RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
- 别名
- RSK2 (RPS6KA3 产品)
- 基因ID
- 6197
- UniProt
- P51812, P18654, D3Z8E0
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
-
You are here:
