Interferon gamma IQ-ELISA 试剂盒
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- 抗原 See all Interferon gamma (IFNG) IQ-ELISA Kits
- Interferon gamma (IFNG)
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适用
- 人
- 检测方法
- qPCR
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Immunoquantitative (PCR-Based) IFN-Gamma ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- 灵敏度
- 3 pg/mL
- 产品特性
- The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA) is an innovative assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human IFN-Gamma IQELISA kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of human IFN-Gamma in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human IFN-Gamma coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and IFN-Gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.
- 组件
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- IFN-Gamma Microplate (Item A): 96 well PCR plate coated with anti-human IFN-Gamma
- Wash Buffer I Concentrate (20x) (Item B): 20x concentrated solution
- Standards (Item C): recombinant human IFN-Gamma
- Assay Diluent A (Item D): diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for IFN-Gamma (Item F): 4x concentrated solution of anti-human IFN-Gamma affinity reagent
- Primer Solution (Item G)
- PCR Master Mix (Item H)
- PCR Preparation buffer (Item I): 10x concentrated buffer
- Final Wash Buffer (Item J): 10x concentrated buffer
- 试剂未包括
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80 °C
- Featured
- Discover our best selling IFNG ELISA Kit
- Top Product
- Discover our top product IFNG ELISA Kit
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Interferon gamma (IFNG) ELISA Kit
IFNG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
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Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鼠 AA 23-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum
Interferon gamma (IFNG) ELISA KitIFNG 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interferon gamma (IFNG) ELISA KitIFNG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: Pig Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- 说明
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Features:
- Excellent for follow up/confirmation of antibody array data
- Pre-coated 96 well plate
- Strip plate format minimizes waste
- Complete kit includes all necessary reagents
- 10x more sensitive than ELISA
- Reproducible and reliable
- Optimized for serum, plasma, urine and cell culture medium
- 1/10th the sample volume of an ELISA
Research Applications:
- Detection of quantitative protein levels in biological fluids
- Validation of antibody array results
- Validation of biomarker discovery studies - 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4 °C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 15μL Primer solution to each well
5. Add 10μL of PCR Master Mix to each well
6. Run real-time PCR - 样品制备
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Recommended Dilution (serum & plasma): 3 - 50 fold
- 实验精密度
- Serum spike tests show recovery is 94.3% with a range of 88.4 to 102.1%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8
- 有效期
- 6 months
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- 抗原 See all Interferon gamma (IFNG) IQ-ELISA Kits
- Interferon gamma (IFNG)
- 别名
- IGN-Gamma (IFNG 产品)
- 别名
- IFG IQ-ELISA Kit, IFI IQ-ELISA Kit, IFN-g IQ-ELISA Kit, Ifg IQ-ELISA Kit, IFNG2 IQ-ELISA Kit, IFN-gamma IQ-ELISA Kit, IFN-G IQ-ELISA Kit, IFNG IQ-ELISA Kit, IFNgamma IQ-ELISA Kit, TCRalpha IQ-ELISA Kit, INF-G IQ-ELISA Kit, ifng IQ-ELISA Kit, interferon gamma IQ-ELISA Kit, interferon, gamma 1-2 IQ-ELISA Kit, IFNG IQ-ELISA Kit, Ifng IQ-ELISA Kit, ifng1-2 IQ-ELISA Kit
- 背景
- Synonyms: Interferon gamma (IFN-gamma) (Immune interferon), IFNG
- 基因ID
- 3458
- UniProt
- P01579
- 途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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