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PDE1C ELISA 试剂盒

PDE1C 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
产品编号 ABIN5674919
发货至: 中国
  • 抗原 See all PDE1C ELISA试剂盒
    PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))
    适用
    • 2
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0042 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all PDE1C ELISA试剂盒
    PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))
    别名
    Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) (PDE1C 产品)
    别名
    CG14940 ELISA Kit, CG14942 ELISA Kit, CG14943 ELISA Kit, CG14944 ELISA Kit, CG31757 ELISA Kit, CG31758 ELISA Kit, CG42325 ELISA Kit, CG44007 ELISA Kit, Dmel\\CG44007 ELISA Kit, Dmel_CG14940 ELISA Kit, Dmel_CG14943 ELISA Kit, Dmel_CG14944 ELISA Kit, Dmel_CG31757 ELISA Kit, Dmel_CG31758 ELISA Kit, Dmel_CG42325 ELISA Kit, PDE1C ELISA Kit, PDE1c ELISA Kit, GB13690 ELISA Kit, Hcam3 ELISA Kit, Phosphodiesterase 1c ELISA Kit, phosphodiesterase 1C ELISA Kit, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A ELISA Kit, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C ELISA Kit, phosphodiesterase 1C, calmodulin-dependent b ELISA Kit, Pde1c ELISA Kit, PDE1C ELISA Kit, LOC724389 ELISA Kit, LOC100019026 ELISA Kit, pde1cb ELISA Kit
    背景
    Alternative name: Hcam3, Cam-PDE 1C, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
    基因ID
    81742
    UniProt
    Q63421
    途径
    EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
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