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NME1 ELISA 试剂盒

NME1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
产品编号 ABIN5674914
发货至: 中国
  • 抗原 See all NME1 ELISA试剂盒
    NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
    适用
    • 3
    • 2
    • 1
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    灵敏度
    0053 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all NME1 ELISA试剂盒
    NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
    别名
    Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) (NME1 产品)
    别名
    AWD ELISA Kit, GAAD ELISA Kit, NB ELISA Kit, NBS ELISA Kit, NDKA ELISA Kit, NDPK-A ELISA Kit, NDPKA ELISA Kit, NM23 ELISA Kit, NM23-H1 ELISA Kit, AL024257 ELISA Kit, NM23-M1 ELISA Kit, NM23A ELISA Kit, NDPK-Z1 ELISA Kit, NM23-B ELISA Kit, NM23-Z1 ELISA Kit, ndpkz1 ELISA Kit, nm23b ELISA Kit, nme1 ELISA Kit, nme2 ELISA Kit, nme2b1 ELISA Kit, NME1 ELISA Kit, NME1-NME2 ELISA Kit, NM23-C1 ELISA Kit, ndpk-b ELISA Kit, ndpkb ELISA Kit, nm23-h2 ELISA Kit, nm23ndk ELISA Kit, puf ELISA Kit, NBR-A ELISA Kit, NBR-B ELISA Kit, NME1-1 ELISA Kit, nm23ndk-a ELISA Kit, NDK I ELISA Kit, NDPK I ELISA Kit, T30A10.80 ELISA Kit, T30A10_80 ELISA Kit, NDP kinase I ELISA Kit, NME/NM23 nucleoside diphosphate kinase 1 ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 ELISA Kit, non-metastatic cells 1, protein (NM23A) expressed in ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 S homeolog ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 L homeolog ELISA Kit, nucleoside diphosphate kinase ELISA Kit, nucleoside diphosphate kinase 1 ELISA Kit, NME1 ELISA Kit, Nme1 ELISA Kit, nme2b.1 ELISA Kit, nme2.S ELISA Kit, nme2.L ELISA Kit, LOC547870 ELISA Kit, NDPK1 ELISA Kit, LOC107790563 ELISA Kit
    背景
    Alternative name: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein
    基因ID
    18102
    UniProt
    P15532
    途径
    Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
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