NME1 ELISA 试剂盒
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- 抗原 See all NME1 ELISA试剂盒
- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- 0053 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) ELISA Kit
NME1 适用: 人 Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) ELISA KitNME1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) ELISA KitNME1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all NME1 ELISA试剂盒
- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
- 别名
- Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) (NME1 产品)
- 别名
- AWD ELISA Kit, GAAD ELISA Kit, NB ELISA Kit, NBS ELISA Kit, NDKA ELISA Kit, NDPK-A ELISA Kit, NDPKA ELISA Kit, NM23 ELISA Kit, NM23-H1 ELISA Kit, AL024257 ELISA Kit, NM23-M1 ELISA Kit, NM23A ELISA Kit, NDPK-Z1 ELISA Kit, NM23-B ELISA Kit, NM23-Z1 ELISA Kit, ndpkz1 ELISA Kit, nm23b ELISA Kit, nme1 ELISA Kit, nme2 ELISA Kit, nme2b1 ELISA Kit, NME1 ELISA Kit, NME1-NME2 ELISA Kit, NM23-C1 ELISA Kit, ndpk-b ELISA Kit, ndpkb ELISA Kit, nm23-h2 ELISA Kit, nm23ndk ELISA Kit, puf ELISA Kit, NBR-A ELISA Kit, NBR-B ELISA Kit, NME1-1 ELISA Kit, nm23ndk-a ELISA Kit, NDK I ELISA Kit, NDPK I ELISA Kit, T30A10.80 ELISA Kit, T30A10_80 ELISA Kit, NDP kinase I ELISA Kit, NME/NM23 nucleoside diphosphate kinase 1 ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 ELISA Kit, non-metastatic cells 1, protein (NM23A) expressed in ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 S homeolog ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 L homeolog ELISA Kit, nucleoside diphosphate kinase ELISA Kit, nucleoside diphosphate kinase 1 ELISA Kit, NME1 ELISA Kit, Nme1 ELISA Kit, nme2b.1 ELISA Kit, nme2.S ELISA Kit, nme2.L ELISA Kit, LOC547870 ELISA Kit, NDPK1 ELISA Kit, LOC107790563 ELISA Kit
- 背景
- Alternative name: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein
- 基因ID
- 18102
- UniProt
- P15532
- 途径
- Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
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