RNLS ELISA 试剂盒
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- 抗原 See all RNLS ELISA试剂盒
- RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- 0.69 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Renalase, FAD-Dependent Amine Oxidase (RNLS) ELISA Kit
RNLS 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Renalase, FAD-Dependent Amine Oxidase (RNLS) ELISA KitRNLS 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Renalase, FAD-Dependent Amine Oxidase (RNLS) ELISA KitRNLS 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Renalase, FAD-Dependent Amine Oxidase (RNLS) ELISA KitRNLS 适用: 大鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Renalase, FAD-Dependent Amine Oxidase (RNLS) ELISA KitRNLS 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all RNLS ELISA试剂盒
- RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))
- 别名
- Renalase (RNLS) (RNLS 产品)
- 别名
- zgc:92286 ELISA Kit, C10orf59 ELISA Kit, RENALASE ELISA Kit, 6530404N21Rik ELISA Kit, AI452315 ELISA Kit, AW060440 ELISA Kit, RGD1309804 ELISA Kit, renalase, FAD dependent amine oxidase ELISA Kit, renalase, FAD-dependent amine oxidase ELISA Kit, RNLS ELISA Kit, rnls ELISA Kit, Rnls ELISA Kit
- 背景
- Alternative name: MAO-C, C10orf60, FAD-Dependent Amine Oxidase, Monoamine oxidase-C, alpha-NAD(P)H oxidase/anomerase
- 基因ID
- 67795
- UniProt
- A7RDN6
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