Transglutaminase 2 ELISA 试剂盒
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- 抗原 See all Transglutaminase 2 (TGM2) ELISA试剂盒
- Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0058 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all Transglutaminase 2 (TGM2) ELISA试剂盒
- Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))
- 别名
- Transglutaminase 2, Tissue (TGM2) (TGM2 产品)
- 别名
- G-ALPHA-h ELISA Kit, GNAH ELISA Kit, TG2 ELISA Kit, TGC ELISA Kit, tgm2 ELISA Kit, zgc:101081 ELISA Kit, TgaseII ELISA Kit, TGM2 ELISA Kit, fc12b04 ELISA Kit, wu:fc12b04 ELISA Kit, zgc:136700 ELISA Kit, TGL2 ELISA Kit, G[a]h ELISA Kit, TGase2 ELISA Kit, tTG ELISA Kit, tTGas ELISA Kit, transglutaminase 2 ELISA Kit, transglutaminase 2, C polypeptide A ELISA Kit, transglutaminase 2b ELISA Kit, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) ELISA Kit, transglutaminase 2, C polypeptide ELISA Kit, TGM2 ELISA Kit, tgm2a ELISA Kit, Tgm2 ELISA Kit, tgm2b ELISA Kit
- 背景
- Alternative name: tTG, TG2, C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase, Transglutaminase C, Transglutaminase H
- 基因ID
- 21817
- UniProt
- P21981
- 途径
- Tube Formation, Thromboxane A2 Receptor Signaling
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