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NEIL3 ELISA 试剂盒

NEIL3 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5674878
发货至: 中国
  • 抗原 See all NEIL3 ELISA试剂盒
    NEIL3 (Nei Endonuclease VIII-Like 3 (NEIL3))
    适用
    • 3
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78.125 pg/mL - 5000 pg/mL
    最低检测浓度
    78.125 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    34.55 pg/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product NEIL3 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all NEIL3 ELISA试剂盒
    NEIL3 (Nei Endonuclease VIII-Like 3 (NEIL3))
    别名
    Nei Endonuclease VIII Like Protein 3 (NEIL3) (NEIL3 产品)
    别名
    RGD1310562 ELISA Kit, NEIL3 ELISA Kit, zgc:103553 ELISA Kit, neil3 ELISA Kit, MAC12.12 ELISA Kit, MAC12_12 ELISA Kit, FGP2 ELISA Kit, FPG2 ELISA Kit, NEI3 ELISA Kit, hFPG2 ELISA Kit, hNEI3 ELISA Kit, AI449477 ELISA Kit, BC034753 ELISA Kit, C85903 ELISA Kit, nei-like DNA glycosylase 3 ELISA Kit, nei like DNA glycosylase 3 ELISA Kit, GRF zinc finger / Zinc knuckle protein ELISA Kit, nei-like DNA glycosylase 3 L homeolog ELISA Kit, nei like 3 (E. coli) ELISA Kit, Neil3 ELISA Kit, NEIL3 ELISA Kit, neil3 ELISA Kit, AT5G13920 ELISA Kit, neil3.L ELISA Kit
    背景
    Alternative name: FPG2, hFPG2, hNEI3, Endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Nei-like protein 3
    基因ID
    55247
    UniProt
    Q8TAT5
    途径
    DNA Damage Repair
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