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LRP5 ELISA 试剂盒

LRP5 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5674833
发货至: 中国
  • 抗原 See all LRP5 ELISA试剂盒
    LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
    适用
    • 2
    • 2
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0114 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all LRP5 ELISA试剂盒
    LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
    别名
    Low Density Lipoprotein Receptor Related Protein 5 (LRP5) (LRP5 产品)
    别名
    Xlrp5 ELISA Kit, bmnd1 ELISA Kit, evr1 ELISA Kit, evr4 ELISA Kit, hbm ELISA Kit, lr3 ELISA Kit, lrp5-A ELISA Kit, lrp7 ELISA Kit, oppg ELISA Kit, ops ELISA Kit, opta1 ELISA Kit, vbch2 ELISA Kit, LRP5 ELISA Kit, LRP6 ELISA Kit, BMND1 ELISA Kit, EVR1 ELISA Kit, EVR4 ELISA Kit, HBM ELISA Kit, LR3 ELISA Kit, LRP7 ELISA Kit, OPPG ELISA Kit, OPS ELISA Kit, OPTA1 ELISA Kit, VBCH2 ELISA Kit, mKIAA4142 ELISA Kit, low-density lipoprotein receptor-related protein 5 ELISA Kit, LDL receptor related protein 5 ELISA Kit, LDL receptor related protein 5 L homeolog ELISA Kit, low density lipoprotein receptor-related protein 5 ELISA Kit, LDL receptor related protein 5 like ELISA Kit, LOC100147353 ELISA Kit, LRP5 ELISA Kit, lrp5.L ELISA Kit, lrp5 ELISA Kit, Lrp5 ELISA Kit, LRP5L ELISA Kit
    背景
    Alternative name: HBM, BMND1, EVR1, EVR4, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2, Osteoporosis Pseudoglioma Syndrome, Exudative Vitreoretinopathy 1
    基因ID
    16973
    UniProt
    Q91VN0
    途径
    WNT signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation
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