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DBP ELISA 试剂盒

DBP 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
产品编号 ABIN5674822
发货至: 中国
  • 抗原 See all DBP ELISA试剂盒
    DBP (D Site of Albumin Promoter (Albumin D-Box) Binding Protein (DBP))
    适用
    • 1
    • 1
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    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0063 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product DBP ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all DBP ELISA试剂盒
    DBP (D Site of Albumin Promoter (Albumin D-Box) Binding Protein (DBP))
    别名
    D Site Of Albumin Promoter Binding Protein (DBP) (DBP 产品)
    别名
    DBP ELISA Kit, DABP ELISA Kit, Gc ELISA Kit, dbp ELISA Kit, tef ELISA Kit, D-box binding PAR bZIP transcription factor ELISA Kit, D site albumin promoter binding protein ELISA Kit, GC, vitamin D binding protein ELISA Kit, D site of albumin promoter (albumin D-box) binding protein L homeolog ELISA Kit, DBP ELISA Kit, Dbp ELISA Kit, GC ELISA Kit, dbp.L ELISA Kit
    背景
    Alternative name: DABP, TaxREB302, Albumin D box-binding protein, Albumin D-element-binding protein, Tax-responsive enhancer element-binding protein 302
    基因ID
    1628
    UniProt
    Q10586
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