DBP
(D Site of Albumin Promoter (Albumin D-Box) Binding Protein (DBP))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.156 ng/mL - 10 ng/mL
最低检测浓度
0.156 ng/mL
应用范围
ELISA
样品类型
Tissue Homogenate
Analytical Method
Quantitative
灵敏度
0063 ng/mL
组件
Pre-coated, ready to use 96-well strip plate Standard (freeze dried) Standard Diluent Detection Reagent A Detection Reagent B Assay Diluent A Assay Diluent B TMB Substhumane Stop Solution Wash Buffer(30 x concenthumane) Plate sealer for 96 wells Instruction manual
试剂未包括
1. Microplate reader with 450 ± 10nm filter. 2. Precision single or multi-channel pipettes and disposable tips. 3. Eppendorf Tubes for diluting samples. 4. Deionized or distilled water. 5. Absorbent paper for blotting the microtiter plate. 6. Container for Wash Solution.
Optimal working dilution should be determined by the investigator.
实验时间
1 - 4.5 h
板类型
Pre-coated
实验流程
1. Prepare all reagents, samples and standards 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃ 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃ 4. Aspirate and wash 3 times 5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃ 6. Aspirate and wash 5 times 7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃ 8. Add 50µL Stop Solution. Read at 450nm immediately.
DBP
(D Site of Albumin Promoter (Albumin D-Box) Binding Protein (DBP))
别名
D Site Of Albumin Promoter Binding Protein (DBP) (DBP 产品)
别名
DBP ELISA Kit, DABP ELISA Kit, Gc ELISA Kit, dbp ELISA Kit, tef ELISA Kit, D-box binding PAR bZIP transcription factor ELISA Kit, D site albumin promoter binding protein ELISA Kit, GC, vitamin D binding protein ELISA Kit, D site of albumin promoter (albumin D-box) binding protein L homeolog ELISA Kit, DBP ELISA Kit, Dbp ELISA Kit, GC ELISA Kit, dbp.L ELISA Kit
背景
Alternative name: DABP, TaxREB302, Albumin D box-binding protein, Albumin D-element-binding protein, Tax-responsive enhancer element-binding protein 302