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Arc ELISA 试剂盒

Arc 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5674809
发货至: 中国
  • 抗原 See all Arc ELISA试剂盒
    Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))
    适用
    • 3
    • 3
    • 2
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78.125 pg/mL - 5000 pg/mL
    最低检测浓度
    78.125 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    31 pg/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all Arc ELISA试剂盒
    Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))
    别名
    Activity Regulated Cytoskeleton Associated Protein (ARC) (Arc 产品)
    别名
    ARC ELISA Kit, arg3.1 ELISA Kit, Arc3.1 ELISA Kit, C86064 ELISA Kit, mArc ELISA Kit, Arg3.1 ELISA Kit, rg3.1 ELISA Kit, ARG3.1 ELISA Kit, activity regulated cytoskeleton associated protein ELISA Kit, activity-regulated cytoskeleton-associated protein ELISA Kit, activity regulated cytoskeletal-associated protein ELISA Kit, ARC ELISA Kit, arc ELISA Kit, Arc ELISA Kit
    背景
    Alternative name: Arg3.1, Activity-regulated gene 3.1 protein homolog
    基因ID
    23237
    UniProt
    Q7LC44
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