ALAS1 ELISA 试剂盒
ALAS1
适用: 小鼠
Colorimetric
Sandwich ELISA
0.312 ng/mL - 20 ng/mL
Tissue Homogenate
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- 抗原 See all ALAS1 ELISA试剂盒
- ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0116 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product ALAS1 ELISA Kit
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Aminolevulinate, delta-, Synthase 1 (ALAS1) ELISA Kit
ALAS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all ALAS1 ELISA试剂盒
- ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))
- 别名
- Aminolevulinate Delta Synthase 1 (ALAS1) (ALAS1 产品)
- 别名
- ALAS ELISA Kit, ALAS3 ELISA Kit, ALASH ELISA Kit, MIG4 ELISA Kit, ALAS-N ELISA Kit, Alas-1 ELISA Kit, Alas-h ELISA Kit, ALAS-H ELISA Kit, ALASN ELISA Kit, ALAS1 ELISA Kit, wu:fb58d01 ELISA Kit, wu:fi12g09 ELISA Kit, 5'-aminolevulinate synthase 1 ELISA Kit, aminolevulinic acid synthase 1 ELISA Kit, alanyl-tRNA synthetase protein ELISA Kit, aminolevulinate, delta-, synthase 1 ELISA Kit, ALAS1 ELISA Kit, Alas1 ELISA Kit, alas1 ELISA Kit, alas1.S ELISA Kit, alaS1 ELISA Kit
- 背景
- Alternative name: ALAS3, ALAS, ALASH, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, 5-aminolevulinate synthase, nonspecific, mitochondrial
- 基因ID
- 11655
- UniProt
- Q8VC19
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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