ARHGEF7 ELISA 试剂盒
ARHGEF7
适用: 小鼠
Colorimetric
Sandwich ELISA
78.125 pg/mL - 5000 pg/mL
Tissue Homogenate
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- 抗原 See all ARHGEF7 ELISA试剂盒
- ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78.125 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78.125 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 34 pg/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product ARHGEF7 ELISA Kit
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rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) ELISA Kit
ARHGEF7 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all ARHGEF7 ELISA试剂盒
- ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))
- 别名
- Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) (ARHGEF7 产品)
- 别名
- BETA-PIX ELISA Kit, COOL-1 ELISA Kit, COOL1 ELISA Kit, Nbla10314 ELISA Kit, P50 ELISA Kit, P50BP ELISA Kit, P85 ELISA Kit, P85COOL1 ELISA Kit, P85SPR ELISA Kit, PAK3 ELISA Kit, PIXB ELISA Kit, cool1 ELISA Kit, p50 ELISA Kit, p85 ELISA Kit, pak3 ELISA Kit, pixb ELISA Kit, ARHGEF7 ELISA Kit, P85spr ELISA Kit, Pak3bp ELISA Kit, Cool ELISA Kit, PIX ELISA Kit, betaPix ELISA Kit, betaPix-b ELISA Kit, betaPix-c ELISA Kit, cool-1 ELISA Kit, mKIAA0142 ELISA Kit, p85Cool1 ELISA Kit, p85SPR ELISA Kit, arhgef7 ELISA Kit, bbh ELISA Kit, bpix ELISA Kit, im:7142367 ELISA Kit, wu:fc35h08 ELISA Kit, zgc:101775 ELISA Kit, Rho guanine nucleotide exchange factor 7 ELISA Kit, Rho guanine nucleotide exchange factor 7 S homeolog ELISA Kit, ankyrin repeat domain 10 ELISA Kit, Rho guanine nucleotide exchange factor (GEF7) ELISA Kit, Rho guanine nucleotide exchange factor (GEF) 7b ELISA Kit, ARHGEF7 ELISA Kit, arhgef7.S ELISA Kit, arhgef7 ELISA Kit, ANKRD10 ELISA Kit, Arhgef7 ELISA Kit, arhgef7b ELISA Kit
- 背景
- Alternative name: PAK3, BETA-PIX, COOL1, P50, P50BP, P85, P85SPR, PIXB, SH3 Domain-Containing Proline-Rich Protein, PAK-Interacting Exchange Factor Beta
- 基因ID
- 54126
- UniProt
- Q9ES28
- 途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
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