LCT ELISA 试剂盒
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- 抗原 See all LCT ELISA试剂盒
- LCT (Lactase (LCT))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0.05 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all LCT ELISA试剂盒
- LCT (Lactase (LCT))
- Abstract
- LCT 产品
- 别名
- LAC ELISA Kit, LPH ELISA Kit, LPH1 ELISA Kit, Lph ELISA Kit, Gm100 ELISA Kit, Lphl ELISA Kit, LCT ELISA Kit, PNGH ELISA Kit, GLH1 ELISA Kit, lac ELISA Kit, lct ELISA Kit, lph ELISA Kit, lph1 ELISA Kit, lactase ELISA Kit, lactase ELISA Kit, lactase, gene 1 L homeolog ELISA Kit, lactase, gene 2 L homeolog ELISA Kit, lactase, gene 2 ELISA Kit, lactase phlorizin hydrolase ELISA Kit, LCT ELISA Kit, Lct ELISA Kit, lct.1.L ELISA Kit, lct.2.L ELISA Kit, lct.2 ELISA Kit, LPH ELISA Kit
- 背景
- Alternative name: LAC, LPH, LPH1, Lactose-Phlorizin Hydrolase
- 基因ID
- 3938
- UniProt
- P09848
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