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FBLIM1 ELISA 试剂盒

FBLIM1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5674740
发货至: 中国
  • 抗原 See all FBLIM1 ELISA试剂盒
    FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))
    适用
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0062 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product FBLIM1 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all FBLIM1 ELISA试剂盒
    FBLIM1 (Filamin Binding LIM Protein 1 (FBLIM1))
    Abstract
    FBLIM1 产品
    别名
    FBLIM1 ELISA Kit, CAL ELISA Kit, FBLP-1 ELISA Kit, FBLP1 ELISA Kit, 2410043F08Rik ELISA Kit, Cal ELISA Kit, Fblp1 ELISA Kit, Gt10 ELISA Kit, migfilin ELISA Kit, migfilin(s) ELISA Kit, filamin binding LIM protein 1 ELISA Kit, filamin binding LIM protein 1 S homeolog ELISA Kit, FBLIM1 ELISA Kit, fblim1.S ELISA Kit, Fblim1 ELISA Kit
    背景
    Alternative name: CAL, FBLP-1, FBLP1, Migfilin, Mitogen-inducible 2-interacting protein
    基因ID
    54751
    UniProt
    Q8WUP2
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