SOCS1 ELISA 试剂盒
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- 抗原 See all SOCS1 ELISA试剂盒
- SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0.14 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all SOCS1 ELISA试剂盒
- SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))
- 别名
- Suppressors Of Cytokine Signaling 1 (SOCS1) (SOCS1 产品)
- 别名
- socs1 ELISA Kit, zgc:91868 ELISA Kit, jab ELISA Kit, cis1 ELISA Kit, ssi1 ELISA Kit, tip3 ELISA Kit, cish1 ELISA Kit, ssi-1 ELISA Kit, socs-1 ELISA Kit, SOCS1 ELISA Kit, LOC100228306 ELISA Kit, socs1b ELISA Kit, CIS1 ELISA Kit, CISH1 ELISA Kit, JAB ELISA Kit, SOCS-1 ELISA Kit, SSI-1 ELISA Kit, SSI1 ELISA Kit, TIP3 ELISA Kit, Cish1 ELISA Kit, Cish7 ELISA Kit, Socs-1 ELISA Kit, suppressor of cytokine signaling 1a ELISA Kit, suppressor of cytokine signaling 1 ELISA Kit, suppressor of cytokine signaling 1 L homeolog ELISA Kit, socs1a ELISA Kit, socs1 ELISA Kit, SOCS1 ELISA Kit, socs1.L ELISA Kit, Socs1 ELISA Kit
- 背景
- Alternative name: CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3
- 基因ID
- 12703
- UniProt
- O35716
- 途径
- JAK/STAT Signaling, Interferon-gamma Pathway, TLR signaling, Response to Growth Hormone Stimulus
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