SF3B3 ELISA 试剂盒
SF3B3
适用: 人
Colorimetric
Sandwich ELISA
0.156 ng/mL - 10 ng/mL
Cell Lysate, Tissue Homogenate
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- 抗原 See all SF3B3 ELISA试剂盒
- SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0065 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product SF3B3 ELISA Kit
-
Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) ELISA Kit
SF3B3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all SF3B3 ELISA试剂盒
- SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))
- 别名
- Splicing Factor 3B Subunit 3 (SF3B3) (SF3B3 产品)
- 别名
- GB11574 ELISA Kit, rse1 ELISA Kit, sap130 ELISA Kit, sf3b130 ELISA Kit, staf130 ELISA Kit, sf3b3 ELISA Kit, 1810061H24Rik ELISA Kit, 5730409A01Rik ELISA Kit, AA409318 ELISA Kit, D8Ertd633e ELISA Kit, RSE1 ELISA Kit, SAP130 ELISA Kit, mKIAA0017 ELISA Kit, ik:tdsubc_2b2 ELISA Kit, wu:fb81f05 ELISA Kit, xx:tdsubc_2b2 ELISA Kit, zgc:55440 ELISA Kit, SF3b130 ELISA Kit, STAF130 ELISA Kit, splicing factor 3b subunit 3 ELISA Kit, splicing factor 3B subunit 3 ELISA Kit, splicing factor 3b, subunit 3, 130kDa ELISA Kit, splicing factor 3b subunit 3 L homeolog ELISA Kit, splicing factor 3b, subunit 3 ELISA Kit, SF3B3 ELISA Kit, LOC550935 ELISA Kit, sf3b3 ELISA Kit, Sf3b3 ELISA Kit, LOC100178056 ELISA Kit, sf3b3.L ELISA Kit
- 背景
- Alternative name: RSE1, SAP130, SF3b130, STAF130, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit
- 基因ID
- 23450
- UniProt
- Q15393
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