OPTN ELISA 试剂盒
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- 抗原 See all OPTN ELISA试剂盒
- OPTN (Optineurin (OPTN))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78.125 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78.125 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 32.4 pg/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product OPTN ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all OPTN ELISA试剂盒
- OPTN (Optineurin (OPTN))
- Abstract
- OPTN 产品
- 别名
- nrp ELISA Kit, fip2 ELISA Kit, hip7 ELISA Kit, hypl ELISA Kit, glc1e ELISA Kit, tfiiia-intp ELISA Kit, ALS12 ELISA Kit, FIP2 ELISA Kit, GLC1E ELISA Kit, HIP7 ELISA Kit, HYPL ELISA Kit, NRP ELISA Kit, TFIIIA-INTP ELISA Kit, Fip2 ELISA Kit, 4930441O07Rik ELISA Kit, fj52f04 ELISA Kit, si:ch211-240l19.3 ELISA Kit, wu:fj52f04 ELISA Kit, zgc:66386 ELISA Kit, zgc:77868 ELISA Kit, optineurin ELISA Kit, optineurin L homeolog ELISA Kit, OPTN ELISA Kit, optn ELISA Kit, Optn ELISA Kit, optn.L ELISA Kit
- 背景
- Alternative name: NRP, FIP2, GLC1E, HIP7, HYPL, TFIIIA-INTP, Glaucoma 1,Open Angle,E(Adult-Onset), Huntingtin-interacting protein 7, Optic neuropathy-inducing protein, E3-14.7K-interacting protein
- 基因ID
- 246294
- UniProt
- Q8R5M4
- 途径
- M Phase
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