HRH1 ELISA 试剂盒
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- 抗原 See all HRH1 ELISA试剂盒
- HRH1 (Histamine Receptor H1 (HRH1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0113 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product HRH1 ELISA Kit
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Histamine Receptor H1 (HRH1) ELISA Kit
HRH1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Histamine Receptor H1 (HRH1) ELISA KitHRH1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Histamine Receptor H1 (HRH1) ELISA KitHRH1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all HRH1 ELISA试剂盒
- HRH1 (Histamine Receptor H1 (HRH1))
- Abstract
- HRH1 产品
- 别名
- H1-R ELISA Kit, hisH1 ELISA Kit, Bphs ELISA Kit, H1R ELISA Kit, HH1R ELISA Kit, Hir ELISA Kit, HRH1 ELISA Kit, H1 ELISA Kit, Hisr ELISA Kit, histamine receptor H1 ELISA Kit, histamine receptor H 1 ELISA Kit, HRH1 ELISA Kit, Hrh1 ELISA Kit, hrh1 ELISA Kit
- 背景
- Alternative name: H1-R, hisH1
- 基因ID
- 15465
- UniProt
- P70174
- 途径
- Regulation of Carbohydrate Metabolic Process
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