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GRIN1/NMDAR1 ELISA 试剂盒

GRIN1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5674688
发货至: 中国
  • 抗原 See all GRIN1/NMDAR1 (GRIN1) ELISA试剂盒
    GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))
    适用
    • 6
    • 5
    • 3
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0122 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all GRIN1/NMDAR1 (GRIN1) ELISA试剂盒
    GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))
    别名
    Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) (GRIN1 产品)
    别名
    GluN1 ELISA Kit, MRD8 ELISA Kit, NMDA1 ELISA Kit, NMDAR1 ELISA Kit, NR1 ELISA Kit, nmda1 ELISA Kit, nmdar1 ELISA Kit, GB19253 ELISA Kit, GRIN1 ELISA Kit, NMD-R1 ELISA Kit, Z16 ELISA Kit, GluRdelta1 ELISA Kit, GluRzeta1 ELISA Kit, M100174 ELISA Kit, Nmdar ELISA Kit, Rgsc174 ELISA Kit, NMDAR1.1 ELISA Kit, zgc:152678 ELISA Kit, glutamate ionotropic receptor NMDA type subunit 1 ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 1 S homeolog ELISA Kit, NMDA receptor 1 ELISA Kit, G protein-regulated inducer of neurite outgrowth 1 ELISA Kit, glutamate receptor, ionotropic, NMDA1 (zeta 1) ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 1a ELISA Kit, GRIN1 ELISA Kit, grin1.S ELISA Kit, grin1 ELISA Kit, Grin1 ELISA Kit, Nmdar1 ELISA Kit, Gprin1 ELISA Kit, grin1a ELISA Kit
    背景
    Alternative name: NMDA1, NMDAR1, NR1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1
    基因ID
    14810
    UniProt
    P35438
    途径
    Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
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