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PLA2G3 ELISA 试剂盒

PLA2G3 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum
产品编号 ABIN5674686
发货至: 中国
  • 抗原 See all PLA2G3 ELISA试剂盒
    PLA2G3 (Phospholipase A2, Group III (PLA2G3))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.781 ng/mL - 50 ng/mL
    最低检测浓度
    0.781 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum
    Analytical Method
    Quantitative
    灵敏度
    0.25 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product PLA2G3 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all PLA2G3 ELISA试剂盒
    PLA2G3 (Phospholipase A2, Group III (PLA2G3))
    Abstract
    PLA2G3 产品
    别名
    PLA2G3 ELISA Kit, GIII-SPLA2 ELISA Kit, SPLA2III ELISA Kit, 9130003P18Rik ELISA Kit, phospholipase A2 group III ELISA Kit, phospholipase A2, group III ELISA Kit, PLA2G3 ELISA Kit, Pla2g3 ELISA Kit
    背景
    Alternative name: GIII-SPLA2, sPLA2-III, Group 3 secretory phospholipase A2, Group III secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase 3
    基因ID
    50487
    UniProt
    Q9NZ20
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