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Apelin Receptor ELISA 试剂盒

APLNR 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Tissue Homogenate
产品编号 ABIN5674667
发货至: 中国
  • 抗原 See all Apelin Receptor (APLNR) ELISA试剂盒
    Apelin Receptor (APLNR)
    适用
    • 2
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.625 pg/mL - 1000 pg/mL
    最低检测浓度
    15.625 pg/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    5.9 pg/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all Apelin Receptor (APLNR) ELISA试剂盒
    Apelin Receptor (APLNR)
    Abstract
    APLNR 产品
    别名
    AGTRL1 ELISA Kit, APJ ELISA Kit, APJR ELISA Kit, HG11 ELISA Kit, Agtrl1 ELISA Kit, msr/apj ELISA Kit, Apj ELISA Kit, X-msr ELISA Kit, Xangio1 ELISA Kit, Xmsr ELISA Kit, agtrl1 ELISA Kit, angio1 ELISA Kit, apj ELISA Kit, apjr ELISA Kit, hg11 ELISA Kit, msr ELISA Kit, APLNR ELISA Kit, DKFZp469C1532 ELISA Kit, agtrl1-B ELISA Kit, agtrl1b ELISA Kit, Agtrl1a ELISA Kit, grn ELISA Kit, zgc:114063 ELISA Kit, APJa ELISA Kit, XAngio1 ELISA Kit, agtrl1-A ELISA Kit, agtrl1a ELISA Kit, aplnr-a ELISA Kit, aplnr-b ELISA Kit, x-MSR ELISA Kit, xAngio1 ELISA Kit, APLNR1 ELISA Kit, apelin receptor ELISA Kit, apelin receptor S homeolog ELISA Kit, apelin receptor b ELISA Kit, apelin receptor L homeolog ELISA Kit, APLNR ELISA Kit, Aplnr ELISA Kit, aplnr ELISA Kit, aplnr.S ELISA Kit, aplnrb ELISA Kit, aplnr.L ELISA Kit
    背景
    Alternative name: AGTRL1, HG11, APJR, APJ, APJ Receptor, Angiotensin II Receptor-Like 1, Angiotensin receptor-like 1
    基因ID
    23796
    UniProt
    Q9WV08
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