YBX1 ELISA 试剂盒
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- 抗原 See all YBX1 ELISA试剂盒
- YBX1 (Y Box Binding Protein 1 (YBX1))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Y-Box Binding Protein 1 (YBX1). No significant cross-reactivity or interference between Y-Box Binding Protein 1 (YBX1) and analogues was observed.
- 灵敏度
- 0.063 ng/mL
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Y Box Binding Protein 1 (YBX1) ELISA Kit
YBX1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Y Box Binding Protein 1 (YBX1) ELISA KitYBX1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Y-Box Binding Protein 1 (YBX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Y-Box Binding Protein 1 (YBX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Y-Box Binding Protein 1 (YBX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Y-Box Binding Protein 1 (YBX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Y-Box Binding Protein 1 (YBX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Y-Box Binding Protein 1 (YBX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all YBX1 ELISA试剂盒
- YBX1 (Y Box Binding Protein 1 (YBX1))
- 别名
- Y-Box Binding Protein 1 (YBX1 产品)
- 别名
- BP-8 ELISA Kit, CSDA2 ELISA Kit, CSDB ELISA Kit, DBPB ELISA Kit, MDR-NF1 ELISA Kit, NSEP-1 ELISA Kit, NSEP1 ELISA Kit, YB-1 ELISA Kit, YB1 ELISA Kit, Byb1 ELISA Kit, Cbfa ELISA Kit, Dbpb ELISA Kit, Ef1a ELISA Kit, Msy1 ELISA Kit, Nsep1 ELISA Kit, Yb1 ELISA Kit, bp-8 ELISA Kit, csda2 ELISA Kit, csdb ELISA Kit, dbpb ELISA Kit, frgy1 ELISA Kit, mdr-nf1 ELISA Kit, nsep-1 ELISA Kit, nsep1 ELISA Kit, yb1 ELISA Kit, 1700102N10Rik ELISA Kit, C79409 ELISA Kit, EF1A ELISA Kit, MSY1 ELISA Kit, dbpB ELISA Kit, mYB-1a ELISA Kit, nuclease sensitive element binding protein 1 ELISA Kit, Y-box binding protein 1 ELISA Kit, Y box binding protein 1 ELISA Kit, Y-box binding protein 1 S homeolog ELISA Kit, nuclease-sensitive element-binding protein 1 ELISA Kit, Y box protein 1 ELISA Kit, nsep1 ELISA Kit, YBX1 ELISA Kit, Ybx1 ELISA Kit, ybx1.S ELISA Kit, LOC479011 ELISA Kit
- 背景
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Gene Name: Y-Box Binding Protein 1
Gene Aliases: BP8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP1, YB1, Nuclease Sensitive Element Binding Protein 1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B
- 途径
- Regulation of Muscle Cell Differentiation
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