ERCC5 CLIA Kit
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- 抗原 See all ERCC5 CLIA Kits
- ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))
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适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 156.25 pg/mL - 10000 pg/mL
- 最低检测浓度
- 156.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Xeroderma Pigmentosum, Complementation Group G (XPG). No significant cross-reactivity or interference between Xeroderma Pigmentosum, Complementation Group G (XPG) and analogues was observed.
- 灵敏度
- 5.51 pg/mL
- Top Product
- Discover our top product ERCC5 ELISA Kit
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DNA Repair Protein Complementing XP-G Cells (ERCC5) ELISA Kit
ERCC5 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Xeroderma Pigmentosum, Complementation Group G (XPG) level in the sample or standard.,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ERCC5 CLIA Kits
- ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))
- 别名
- Xeroderma Pigmentosum, Complementation Group G (ERCC5 产品)
- 别名
- COFS3 CLIA Kit, ERCM2 CLIA Kit, UVDR CLIA Kit, XPG CLIA Kit, XPGC CLIA Kit, cofs3 CLIA Kit, ercm2 CLIA Kit, uvdr CLIA Kit, xpg CLIA Kit, xpgc CLIA Kit, Xpg CLIA Kit, ERCC excision repair 5, endonuclease CLIA Kit, excision repair cross-complementation group 5 L homeolog CLIA Kit, excision repair cross-complementing rodent repair deficiency, complementation group 5 CLIA Kit, ERCC5 CLIA Kit, ercc5.L CLIA Kit, Ercc5 CLIA Kit
- 背景
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Gene Name: Xeroderma Pigmentosum, Complementation Group G
Gene Aliases: ERCM2, ERCC5, UVDR, XPGC, Excision Repair Cross-Complementing Rodent Repair Deficiency 5, Cockayne Syndrome, DNA repair protein complementing XP-G cells
- 基因ID
- 2073
- UniProt
- P28715
- 途径
- DNA Damage Repair
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