WFS1 ELISA 试剂盒
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- 抗原 See all WFS1 ELISA试剂盒
- WFS1 (Wolfram Syndrome 1 (WFS1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Wolfram Syndrome Protein 1 (WFS1). No significant cross-reactivity or interference between Wolfram Syndrome Protein 1 (WFS1) and analogues was observed.
- 灵敏度
- 0.133 ng/mL
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Wolfram Syndrome 1 (WFS1) ELISA Kit
WFS1 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Wolfram Syndrome 1 (WFS1) ELISA KitWFS1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wolfram Syndrome Protein 1 (WFS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wolfram Syndrome Protein 1 (WFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wolfram Syndrome Protein 1 (WFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wolfram Syndrome Protein 1 (WFS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wolfram Syndrome Protein 1 (WFS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wolfram Syndrome Protein 1 (WFS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all WFS1 ELISA试剂盒
- WFS1 (Wolfram Syndrome 1 (WFS1))
- 别名
- Wolfram Syndrome Protein 1 (WFS1 产品)
- 别名
- WFRS ELISA Kit, WFS ELISA Kit, WFSL ELISA Kit, CG4917 ELISA Kit, Dmel\\CG4917 ELISA Kit, GB15257 ELISA Kit, WFS1 ELISA Kit, AI481085 ELISA Kit, wolframin ELISA Kit, wfs1 ELISA Kit, wolframin ER transmembrane glycoprotein ELISA Kit, wolfram syndrome 1 ELISA Kit, wolframin ELISA Kit, Wolfram syndrome 1 (wolframin) L homeolog ELISA Kit, Wfs1 ELISA Kit, WFS1 ELISA Kit, wfs1 ELISA Kit, LOC552818 ELISA Kit, CpipJ_CPIJ013087 ELISA Kit, wfs1.L ELISA Kit
- 背景
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Gene Name: Wolfram Syndrome Protein 1
Gene Aliases: DFNA14, DFNA38, DFNA6, DIDMOAD, WFRS, WFS, Wolframin
- 途径
- Sensory Perception of Sound, Carbohydrate Homeostasis, ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome
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